Journal: International Journal of Molecular Sciences

Article Title: Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

doi: 10.3390/ijms25094625

Figure Lengend Snippet: Lectins used in microarray; their glycan specificity and reactivity with a PD transferrin (weak “+” and strong “++”).

Article Snippet: A microarray analysis was performed with each sample separately using biotinylated lectins, epoxy microarray slides (NEXTERION Slide E, Schott, Germany), a non-contact piezoelectric sciFLEXARRAYER S1 microarray spotter and a piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany) [ ].

Techniques: Microarray, Glycoproteomics, Whole Genome Amplification

Journal: International Journal of Molecular Sciences

Article Title: Glycosylation and Characterization of Human Transferrin in an End-Stage Kidney Disease

doi: 10.3390/ijms25094625

Figure Lengend Snippet: Reactivity of transferrin isolated from healthy persons (H) and patients on peritoneal dialysis (PD) with lectins. A statistically significant difference between groups ( p < 0.05) is labeled with “*”.

Article Snippet: A microarray analysis was performed with each sample separately using biotinylated lectins, epoxy microarray slides (NEXTERION Slide E, Schott, Germany), a non-contact piezoelectric sciFLEXARRAYER S1 microarray spotter and a piezo dispense capillary PDC 80 (Scienion AG, Berlin, Germany) [ ].

Techniques: Isolation, Labeling

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: Schematic representation of intrinsic and extrinsic factors influencing microarray analysis of CBP–carbohydrate interactions. The color coding in the background from red to white (from top right to bottom left) indicates the extent of research work assessing these parameters.

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: 4D approach adopted to optimize factors contributing to variability in microarray analysis of CBP–carbohydrate interactions. The chronological order of the four major steps in optimization follows the solid arrow heads from Design to Dispense, Detect and Deduce.

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: The effect of blocking buffer composition on signal-to-noise-ratio values of the interaction of lectin ConA with serum and fetuin printed on the microarray slide (1-drop volume, 50 μg/mL). Error bars indicate standard error ( n = 10). The microarray slides were blocked with the eight buffers described in .

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Blocking Assay, Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: The effect of blocking time on the signal intensities of the interactions of lectin ConA with serum and fetuin printed on the microarray slide (1-drop volume, 50 μg/mL). Error bars indicate standard error ( n = 10).

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Blocking Assay, Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: The effect of lectin incubation times on the signal intensities of the interaction of lectin ConA with serum (green bars) and fetuin (orange bars) printed on the microarray slide (1-drop volume, 100 μg/mL). Error bars indicate standard error ( n = 10).

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Incubation, Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: The effect of dye incubation times on signal intensities of the interaction of lectin ConA with serum (green bars and fetuin (orange bars) printed on microarray slide (1-drop volume, 100 μg/mL). Error bars indicate standard error ( n = 10).

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Incubation, Microarray

Journal: Sensors (Basel, Switzerland)

Article Title: A Holistic 4D Approach to Optimize Intrinsic and Extrinsic Factors Contributing to Variability in Microarray Biosensing in Glycomics

doi: 10.3390/s23125362

Figure Lengend Snippet: Relative intensity values of interaction of lectin ConA with serum printed on microarray slide (-drop volume, 100 μg/mL) at storage conditions (1) after 8 h at 25 °C; (2) after 24 h at −20 °C, blocked and sealed; (3) after 24 h at −20 °C, sealed without blocking; (4) after 24 h at 4 °C, without blocking or vacuum sealing. Error bars indicate standard error ( n = 10).

Article Snippet: The microarray slides with epoxysilane coating NEXTERION ® E were bought from SCHOTT MINIFAB (Jena, Germany).

Techniques: Microarray, Blocking Assay